Join the team!
Positions in the lab provide the opportunity to work alongside experts in many disciplines on projects directly connected to clinical data and pre-clinical modeling. As such, communication skills and the ability to work well in a team setting will be highly emphasized. Candidates will have the opportunity to contribute to research projects and to gain authorship on research manuscripts. As the laboratory serves as an academic training environment, recent undergraduates seeking research experience and mentorship prior to applying for graduate or medical school are strongly encouraged to apply.
Research Assistant II in cell line engineering
The Lin lab is seeking talented molecular biologists to work in an interdisciplinary team environment on understanding how gene regulation is altered in cancer. This position will involve developing, optimizing, and validating genome editing technologies. These methods will include CRISPR-Cas9 technologies for genome editing and genetic perturbation as well as lentivirus production and transduction for the generation of engineered mammalian cell lines. Candidates will be expected to drive technology development and optimization with minimal supervision and to be able to troubleshoot as needed. Candidates should be able to design experimental approaches with an understanding of how gene regulation is disordered in cancer, how it can be targeted, and the consequences of target perturbation on cell state.
Essential tasks and duties:
Develop, optimize, and run experimental protocols for CRISPR/Cas9 mediated endogenous tagging and/or knockout of genes.
Develop, optimize, and run experimental protocols for lentivirus generation and transduction.
Generate CRISPR/lentiviral constructs via molecular cloning techniques including standard restriction, Gateway and Gibson technologies.
Clearly communicate (oral and written) experimental results and progress.
Annotate and maintain collaborative protocol repositories for all lab members
Work collaboratively with chemical and molecular biologists to engineer mammalian cell lines that can be used to study the mechanism of gene regulation in cancer.
To apply for this position, please see official posting at here
Research Technician II in molecular biology
The Lin lab is seeking molecular biologists to work in an interdisciplinary team environment on understanding how gene regulation is altered in cancer. This position will involve supporting ongoing research in the lab and will include molecular cloning, basic molecular biology techniques, and assisting with maintaining lab stocks, reagents, equipment, and common spaces. Individuals with strong communication and organizational skills with a desire to pursue careers in life sciences are encouraged to apply.
Essential tasks and duties:
Work under the supervision of molecular biologists to design recombinant DNA constructs for cell line engineering.
Generate recombinant DNA constructs via molecular cloning techniques including standard restriction, Gateway and Gibson technologies.
Perform nucleic acid and protein extractions from mammalian cell lines
Perform nucleic acid quantification techniques including gel electrophoresis and real-time PCR
Perform mammalian cell culture
Clearly communicate and document (oral and written) experimental results and progress.
Maintain laboratory stocks, equipment and common spaces.
To apply for this position, please see official posting at here.